Pretreatment with DRE and standard drug (OMP) significantly increased the GSH level compared with EtOH treated group. to the unfavorable control group. DRE conserved glutathione (GSH) levels, reduced malondialdehyde (MDA) levels, and enhanced catalase (CAT) and superoxide dismutase (SOD) enzyme levels. The present study shows that DRE possess protective effects against ethanol-induced ulcer damage in the stomach of rats, which could be attributed to its antioxidant activity. 1. Background Peptic ulcer disease (PUD) is usually CYT387 sulfate salt a gastrointestinal tract disorder, affecting many people globally [1] with the most prevalent type being gastric ulcer. Apart from the main etiological agent which is usually antioxidant biomolecules and enzymes, and gastric acid oversecretion [3]. Alcohol abuse has been associated with gastric ulcers [4]. A systematic review published on this subject backs the assertion that excessive alcohol intake mediates the generation of reactive oxygen species, a known indicator of the disorder [5]. Though there are some antigastric ulcer medications such as the proton pump inhibitors (PIs) and histamine 2-receptor antagonists, the majority leave in their wake inimical effects such as bloating, diarrhea, shortness of breath, fatigue, nausea, dizziness, lactic acidosis, hepatotoxicity, kidney toxicity, and lactic acid intoxication thus limiting their usage [6]. Currently, the mission to CYT387 sulfate salt unearth option and more efficient Rabbit polyclonal to CLOCK treatment therapies is usually imminent due to the fact that many natural bioactive compounds such as flavonoids and alkaloids had been isolated from medicinal plants and have been identified as potential antiulcer brokers [7, 8]. (antimicrobial activity [10C12], antiradical effects, and H+/K+-ATPase inhibitory potential [13] and is nontoxic at 500?mg/kg bwt [14]. Although this herb is used in traditional medicine practices (TMPs) to manage peptic ulcer, there is scanty data to substantiate this ethnopharmacological relevance. This current study sought to evaluate the gastroprotective effect of a flavonoid-rich extract of and identify possible antioxidant biomolecules interplaying in an ethanol-induced ulcer model. Open in a separate window Physique 1 Structures of glycosylflavones compounds, (a) isoorientin (luteolin-6-C-powder and dichloromethane solvent in a flask. The flask was tightly corked and put on an IKA? KS260 basic shaker at a velocity of 200?rpm for 2 days. The mixture was filtered into a 2000?mL flask. After drying the residue at 40C on a water bath, aqueous methanol (70%) was added, corked well, and put on a shaker at 200?rpm for another 2 days. The resultant mixture was later filtered. The filtrate was afterwards concentrated and dried at 40C to get a brown coffee-colored extract of extract (DRE). The crude DRE of 8.3% yield was stored in a freezer at ?20C until ready for use. 2.2. Animals Used in This Experiment Sprague Dawley (SD) rats of either sex (200C250?g) were used for this work. All experiments were conducted in accordance with Principles of laboratory animal care (NIH publication no. 86C23, revised 1985) in accordance with the National Institute of Health Guidelines for the Care and Use of Laboratory Animals [15]. Also, all protocols used in the study were approved by the Departmental Animal Ethics Committee. The animals used for the toxicity study were kept in stainless steel cages with softwood shavings as bedding material whereas the animals used for the ulcer experiment were kept individually in metabolic cages. All cages were kept under ambient heat conditions (24??2C), relative humidity (60C70%), and 12?h light/dark cycle, and water and rat chow were available ad libitum. All animals used in this study were allowed to acclimatize to their new environment for at least two weeks with adequate water and food before the start of the experiment. However, preceding oral administration of DRE and standard drug-omeprazole, the animals were fasted for 24 hours overnight but were still allowed free access to clean water. 2.3. Acute Toxicity Assessment The median lethal dose (LD50) of the extract was determined according to CYT387 sulfate salt the method described by Ansah et al. [14]. DRE at the highest dose of 5000?mg/kg was used for the acute oral toxicity experiment. About sixty rats consisting of 30 male and 30 female rats each were placed into 6 groups of 10 rats each with an equal number of both sexes in each group. Group 1 that acted as normal control was administered distilled water only. Groups 2, 3, 4, 5, and 6 were given extracts at 10, 100,.Background Peptic ulcer disease (PUD) is a gastrointestinal tract disorder, affecting many people globally [1] with the most prevalent type being gastric ulcer. Gross examinations of the stomach lining and histological analysis of gastric lesions were carried out coupled with an assessment of the antioxidant activity of gastric mucosa using MDA, GSH, CAT, and SOD as indicators. The data suggested a significant attenuation in gastric mucosal damage in DRE-pretreated ethanol-induced gastric ulcer reflected in the antioxidant status. There was also a reduction or absence of hemorrhage, edema, and leucocytes infiltration in DRE-treated groups compared to the negative control group. DRE conserved glutathione (GSH) levels, reduced malondialdehyde (MDA) levels, and enhanced catalase (CAT) and superoxide dismutase (SOD) enzyme levels. The present study shows that DRE possess protective effects against ethanol-induced ulcer damage in the stomach of rats, which could be attributed to its antioxidant activity. 1. Background Peptic ulcer disease (PUD) is a gastrointestinal tract disorder, affecting many people globally [1] with the most prevalent type being gastric ulcer. Apart from the main etiological agent which is antioxidant biomolecules and enzymes, and gastric acid oversecretion [3]. Alcohol abuse has been associated with gastric ulcers [4]. A systematic review published on this subject backs the assertion that excessive alcohol intake mediates the generation of reactive oxygen species, a known indicator of the disorder [5]. Though there are some antigastric ulcer medications such as the proton pump inhibitors (PIs) and histamine 2-receptor antagonists, the majority leave in their wake inimical effects such as bloating, diarrhea, shortness of breath, fatigue, nausea, dizziness, lactic acidosis, hepatotoxicity, kidney toxicity, and lactic acid intoxication thus limiting their usage [6]. Currently, the quest to unearth alternative and more efficient treatment therapies is imminent due to the fact that many natural bioactive compounds such as flavonoids and alkaloids had been isolated from medicinal plants and have been identified as potential antiulcer agents [7, 8]. (antimicrobial activity [10C12], antiradical effects, and H+/K+-ATPase inhibitory potential [13] and is nontoxic at 500?mg/kg bwt [14]. Although this plant is used in traditional medicine practices (TMPs) to manage peptic ulcer, there is scanty data to substantiate this ethnopharmacological relevance. This current study sought to evaluate the gastroprotective effect of a flavonoid-rich extract of and identify possible antioxidant biomolecules interplaying in an ethanol-induced ulcer model. Open in a separate window Figure 1 Structures of glycosylflavones compounds, (a) isoorientin (luteolin-6-C-powder and dichloromethane solvent in a flask. The flask was tightly corked and put on an IKA? KS260 basic shaker at a speed of 200?rpm for 2 days. The mixture was filtered into a 2000?mL flask. After drying the residue at 40C on a water bath, aqueous methanol (70%) was added, corked well, and put on a shaker at 200?rpm for another 2 days. The resultant mixture was later filtered. The filtrate was afterwards concentrated and dried at 40C to get a brown coffee-colored extract of extract (DRE). The crude DRE of 8.3% yield was stored in a freezer at ?20C until ready for use. 2.2. Animals Used in This Experiment Sprague Dawley (SD) rats of either sex (200C250?g) were used for this work. All experiments were conducted in accordance with Principles of laboratory animal care (NIH publication no. 86C23, revised 1985) in accordance with the National Institute of Health Guidelines for the Care and Use of Laboratory Animals [15]. Also, all protocols used in the study were approved by the Departmental Animal Ethics Committee. The animals used for the toxicity study were kept in stainless steel cages with softwood shavings as bedding material whereas the animals used for the ulcer experiment were kept individually in metabolic cages. All cages were kept.