The following day time, samples were rinsed and washed three times for 15?min with PBST, incubated for 1?h and 15?min with secondary antibodies, washed three times for 15?min with PBST and once with 1PBS
The following day time, samples were rinsed and washed three times for 15?min with PBST, incubated for 1?h and 15?min…