If the specimen does not contain antibodies, a red line will not appear in this region, indicating a negative result. injected with heat-killed treponemes suspended in blood every 12 hours. The detection of IgG/IgM was based on the basic principle of double antigen sandwich immunoassay, in which purified recombinant antigens are employed sufficiently to identify antibodies to Syphilis. The outcomes of interest included the proportion of Syphilis positive rats and the maximal survival hours of in banked blood. Results: 14 rats (77.8%) out of the 18 rats that were involved in group A developed orchitis and positive serology up to 72 hours of storage time, p 0.05. 2 rats (25%) in group B developed orchitis after 72hrs of storage time. All the 18 rats (100%) in the control group C and D showed neither medical nor serological changes. Conclusion: It was concluded that the survival time of in banked donor blood lies between 72-120hrs with this study. No matter blood banking temp, and additional transfusion transmissible infections should be screened for prior to allogeneic transfusion. spirochaetes are fragile, and cannot withstand blood-bank temp when subjected to it for long hours. Hence, screening is not carried out on donated devices of blood before allogeneic transfusion despite World Health Corporation (W.H.O.) recommendations. However, in some intense emergencies, some donated devices of blood that has not been previously screened for syphilis is probably not banked whatsoever before transfusion, therefore, putting the recipient at high risk of Syphilis illness. Every year, millions of people are exposed to avoidable, life-threatening risks through the transfusion of unsafe blood. As per a global database, 6 million of 81 million devices of blood collected an-nually in 178 countries are not screened for trans-fusion-transmissible infections[1]. The provision of safe and efficacious blood and blood parts for transfusion or developing use involves a number of processes, from the selection of blood donors and the collection, processing and screening of blood donations to the screening of individual samples, the issue of compatible blood and its administration to the patient. There is a risk of error in each process with this transfusion chain and a failure at any of these phases can have severe implications for the recipients of blood and blood products. Therefore, while blood transfusion can be life-saving, you will find associated risks, particularly the transmission of blood-borne infections[2]. The microbial providers of importance to blood transfusion solutions are those that are Vegfc transmissible by blood transfusion and may cause morbidity and mortality in recipients. In order to be transmissible by blood, the infectious agent or illness usually has the following characteristics: presence in the blood for long periods; sometimes in high titers, stability in blood stored at 4C or lower, very long incubation period before the appearance of medical signs, asymptomatic phase or only slight symptoms in the blood donor, hence not identifiable during the blood donor selection process[2]. Donated blood is tested by many methods, but the core tests recommended from the World Health Corporation are these four: Hepatitis B Surface WHI-P97 Antigen, antibody to Hepatitis C, antibody to HIV; usually subtypes 1 and 2, serologic test for Syphilis. WHO reported in 2006 that WHI-P97 56 out of 124 countries surveyed did not use these fundamental checks on all blood donations[3]. Syphilis is definitely a sexually transmitted infection (STI) caused by the spirochete. The route of transmission of syphilis is almost constantly by sexual contact, although there may be congenital syphilis via transmission from mother to child in-utero. Syphilis may also be transmitted via blood and blood products, and intravenous WHI-P97 drug use[4]. If not treated, syphilis can cause serious effects.